NADH Dehydrogenase Assay Kit

NADH Dehydrogenase Assay Kit

Product Information

Product Overview:

NADH dehydrogenase (also known as cytochrome c reductase or NADH oxidoreductase) is another important respiratory chain linked mitochondrial enzyme that catalyzes transdehydrogenations between various pyridine nucleotides. This enzyme is known to be inactivated by oxidative stress. Hydroxyl radicals and superoxide anions were also shown to inactivate this enzyme by 60 and 40% respectively in the mitochondrial fraction. NADH dehydrogenase is measured at 340 nm in the presence of NADH and cytochrome C. The rotenone sensitive dehydrogenase activity (NADH oxidase) is assayed in the presence of rotenone. The assay procedure uses 1-ml assay reaction volume, and requires quartz cuvettes of 1 ml capacity and a spectrophotometer with kinetic function and equipped with a temperature control capable of maintaining 370C.


NADH Dehydrogenase Assay Kit includes:

1. NADH Dehydrogenase Assay Buffer
2. NADH Dehydrogenase Lysis Buffer
4. Rotenone
5. Cytochrome C
6. NADH Dehydrogenase Positive Control

Catalog#: ND-5022K
LID#: 226A
Order by Phone or Fax: 1.800.316.3081
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Required Equipment1. Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range at 340 nm, and a temperature control capable of maintaining 370C.
2. Quartz cuvettes capable of reading 1ml reaction volume in a 1-cm light path.
3. Cell sonicator or disrupter for cell lysate preparation and a waring blender for tissue sample homogenization.
MSDS AvailabilityYes, upon request.
Unit Size100 REACTIONS
Country of OriginUnited States of America
Safety StatementsFor research use only. Not for use in diagnostic procedures.
Sample AvailabilityNo

Shipping & Handling

Estimated Ship Time: 3 Business Days

Shipped In: Dry Ice

Hazardous Material: No


Storage Temperature: -20C

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