Glutathione Reductase Assay Kit

Glutathione Reductase Assay Kit

Product Information

Product Overview:

Glutathione reductase is a critical enzyme of the glutathione system that maintains the redox pool of glutathione by reducing oxidized glutathione utilizing NADPH. Glutathione is the major redox buffer of the cell that provides critical protection against oxidative stress. Reduced glutathione is an electron donor for glutathione peroxidase that catalyzes the reduction of peroxides. In this process reduced glutathione is oxidized and is reduced by glutathione reductase using reducing equivalents from NADPH. Therefore, the activity of glutathione reductase is an important indicator of maintenance of cellular redox pool.


In the presence of the enzyme, hydrogen is transferred from NADPH to GSSG, and the reaction is measured spectrophotometrically at 340nm.


Glutathione Reductase Assay includes:
1. Lysis buffer
2. Glutathione Reductase Assay Buffer
4. Glutathione reductase (0.22U/µl, store in aliquots in 2-8oC)

Catalog#: GD-7022K
LID#: 222A
Order by Phone or Fax: 1.800.316.3081
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Required Equipment1. Spectrophotometer capable kinetics function. Capable of reading wavelength of 340 nm and thermostated to 25oC.
2. Quartz cuvettes capable of holding 1.0mL volume.
3. Pipettes capable of measuring 0.05mL, 0.10mL, and 0.550mL pipette tips (disposable).
MSDS AvailabilityYes, upon request.
Unit Size100 REACTIONS
Country of OriginUnited States of America
Safety StatementsFor research use only. Not for use in diagnostic procedures.
Sample AvailabilityNo

Shipping & Handling

Estimated Ship Time: 3 Business Days

Shipped In: Dry Ice

Hazardous Material: No


Storage Temperature: -20C

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