Fluorescein (FITC) Chrompure Human ...

Fluorescein (FITC) Chrompure Human IgG, Fc fragment

Jackson ImmunoResearch Laboratories

Specifications

TargetHuman
Preservative0.05% Sodium Azide
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Amax492
Emax520nm
Conjugate DescriptionFITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is still a widely used fluorophore due to its long history. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium. A better choice for many applications involving FITC is Alexa Fluor® 488 because it is brighter and more photostable.
Physical FormFreeze-dried powder
Grade/PurityBased on immunoelectrophoresis at an antigen concentration of 20 mg/ml, the pattern of precipitation against goat anti-human whole serum is the same as that against goat anti-human IgG, Fc fragment specific. No precipitin line was detected against goat anti-human IgG, F(ab')2 fragment specific; rabbit anti-human IgG, F(ab')2 fragment specific; goat anti-human IgM, Fc5m fragment specific; or goat anti-human IgA, a chain specific.
ConjugationFluorescein (FITC)
SpecificityIgG, Fc fragment specific
Storage Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Unit Size1.0 mg
Safety StatementsFor Research Use Only.
Sample AvailabilityNo

Shipping & Handling

Estimated Ship Time: 3 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Storage Temperature: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol ( ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.

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