Product Overview:
picoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. picoLUCENT™ PLUS-HRP reagents are available in five sizes suitable for 5, 25, 50, 125 and 250 blots as each 4ml of working solution is suitable for 1 mini blot (8 x 7.5cm) In addition, picoLUCENT™ PLUS-HRP is also supplied in a kit format, containing our non-animal protein blocking agent (NAP-BLOCKER™) and wash buffer (femto-TBST™). The picoLUCENT™ PLUS-HRP kits allow detection of low picogram levels (10-12) of antigens with low noise (signal/background) ratio. The kit reagents are sufficient for 25 mini blots or 1,500cm2 of PVDF or nitrocellulose membrane. For a complete chemiluminescence immunodetection system, we offer optional alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat.
Features:
Low picogram detection (10-12) Low Background : High signal ratio 1 year shelf life Reagent for 25, 50 and 125 mini blots Nitrocellulose or PVDF compatible Kit formats supplied with optional HRP or AP conjugated secondary antibodies.