Product Overview:
picoLUCENT™ PLUS-AP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. picoLUCENT™ PLUS-AP is also supplied in a kit format, containing our non-animal protein blocking agent (NAP-BLOCKER™) and wash buffer (femto-TBST™). The picoLUCENT™ PLUS-AP kits allow detection of low picogram levels (10-12) of antigens with low noise (signal/background) ratio. The kit reagents are sufficient for 25 mini blots or 1,500cm2of PVDF or nitrocellulose membrane. The trial size is suitable for 5 mini blots or 300cm2. For a complete chemiluminescence immunodetection system, we offer optional alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat.
Features:
Low picogram detection (10-12) Low Background : High signal ratio 1 year shelf life Reagent for 200cm2and =1,000cm2membrane Nitrocellulose or PVDF compatible Supplied with optional HRP or AP conjugated secondary antibodies.