femtoLUCENT™ PLUS HRP Kit

femtoLUCENT™ PLUS HRP Kit

Product Information

Product Overview:

femtoLUCENT™ PLUS is a femtogram level sensitive chemiluminescence immunodetection system that allows users the option to detect even hard-to-detect low abundance proteins. This immunodetection system is based on an innovative formulation of luminol and 1, 2 dioxetane, a supersensitive detection reagent for HRP (horseradish peroxidase). The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. femtoLUCENT™ PLUS-HRP reagents are available in three sizes suitable for 5, 25, 50 and 125 blots as each 4ml of working solution is suitable for 1 mini blot (8 x 7.5cm) In addition, femtoLUCENT™ PLUS-HRP is also supplied in a kit format, containing our non-animal protein blocking agent (NAP BLOCKER™) and wash buffer (femto-TBST™). The femtoLUCENT™ PLUS-HRP kits allow detection of low femtogram levels (10-15) of antigens with low noise (signal/background) ratio. The kit reagents are sufficient for 25 mini blots or 1,500cm2 of PVDF or nitrocellulose membrane. For a complete chemiluminescence immunodetection system, we offer optional alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat.

Application:

Quick and sensitive non-isotopic detection of proteins on transfer membranes (suitable for nitrocellulose & PVDF membranes) Immunodetection of proteins and antigens for Western blots and dot blot applications Low femtogram detection (10-15) that allows for detection of >10fg protein on a dot blot and >1pg on a Western blot

Features:

Reagents alone or kit formats Intense light emission with low background and high signal Supplied with a novel blocking agent to allow a rapid blocking step that produces a clear background Economical: greater value compared to similar products

Catalog#: 786-10
LID#: 4060P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionFor Western blot development using HRP conjugates
Unit Size100ml kit
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymECL, hrp, ap, protein detection, chemiluminescence, proteins, western blotting, western blots, horseradish peroxidase, alkaline phosphatase, western protein, electrophoresis

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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