femtoLUCENT™ PLUS AP Kit

femtoLUCENT™ PLUS AP Kit

Product Information

Product Overview:

The femtoLUCENT™ PLUS-AP kit is based on alkaline phosphatase (AP) chemiluminescence reaction. The chemiluminescence light emission can be recorded by a short exposure to blue-light sensitive autoradiography films. The femtoLUCENT™ PLUS-AP kit allows for detection of femtogram quantity of antigens with no background. The kit reagents are sufficient for 25 mini blots (8.5 x 7.5 cm size). In addition, femtoLUCENT™ PLUS-AP is also supplied in a kit format, containing our non-animal protein blocking agent (NAP BLOCKER™) and wash buffer (femto-TBST™). The femtoLUCENT™ PLUS-AP kits allow detection of low femtogram levels (10-15) of antigens with low noise (signal/background) ratio. The kit reagents are sufficient for 25 mini blots or 1,500cm2of PVDF or nitrocellulose membrane. The trial size (Cat. # 786-10APT) is suitable for 5 mini blots or 300cm2. For a complete chemiluminescence immunodetection system, we offer optional alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat.

Application:

Quick and sensitive non-isotopic detection of proteins on transfer membranes (suitable for nitrocellulose & PVDF membranes) Immunodetection of proteins and antigens for Western blots and dot blot applications Low femtogram detection(10-15)that allows for detection of >10fg protein on a dot blot and >1pg on a Western blot

Features:

Intense light emission with low background and high signal Supplied with a novel blocking agent to allow a rapid blocking step that produces a clear background Economical: greater value compared to similar products

Catalog#: 786-10AP
LID#: 4072P
Quantity :
Order by Phone or Fax: 1.800.316.3081
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Specifications

Warehouse Storage Temp4C
DescriptionFor Wesern blot development using AP conjugates
Unit Size100ml kit
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymECL, ap, protein detection, chemiluminescence, proteins, western blotting, western blots, alkaline phosphatase, western protein, electrophoresis

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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