Product Overview:
femtoCHROMO™AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X) washing buffer, femtoTBST™Buffer. In addition, optional AP labeled secondary antibodies against mouse and/or rabbit antibodies are supplied. femtoCHROMO™APuses a modified BCIP (5-Bromo-4-Chloro-3'-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.
Features:
Detects >5ng Ready-to-use Single detection step High signal to background ratio High reproducibility