Zymogram Development Buffer [10X] (...

Zymogram Development Buffer [10X] (0.5M Tris.HCl, 2M NaCl, 50mM CaCl2, 0.2% Brij®-35, pH 7.5)

Product Information

Product Overview:

Zymography is an electrophoretic technique, based on SDS-PAGE, that includes an enzyme substrate copolymerized with the polyacrylamide gel. Samples are prepared in Zymogram Sample Buffer without boiling to preserve the structure and activity of the enzyme. Following electrophoresis, the SDS is removed from the gel by washing in Zymogram Renature Buffer that contains a non-ionic detergent. The gels are then equilibrated in Zymogram Developing Buffer, which contains the divalent metal cation required for enzymatic activity. The zymogram is subsequently stained (commonly with Amido Black or Coomassie Brilliant Blue), and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme. • Zymogram Sample Buffer [2X]: 62.5mM Tris, 4% SDS, 25% Glycerol, 0.01% Bromophenol Blue, pH 6.8 • Zymogram Renature Buffer [10X]: 25% Triton ® X-100 • Zymogram Development Buffer [10X]: 0.5M Tris, 2M NaCl, 50mM CaCl2, 0.2% Brij ® 35, pH 7.5

Features:

0.5M Tris.HCL, 2M NaCl, 50mM CaCl2, 0.2% Brij ®-35, pH 7.5

Catalog#: 786-481
LID#: 4447P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage TempRT
DescriptionBuffers for running and loading denaturing, native, tricine and IEF gels. Zymogram related buffers are also offered.
Unit Size125ml
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymZymogram Development Buffer

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: RT

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