Zinc Chelating Resin

Product Information

Product Overview:

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Zinc Chelating Resin is specifically designed for the purification of proteins that associate with Zinc ions (2-3), including 6x histidine tagged proteins. Immobilized metal affinity chromatography (IMAC) resin utilizingzinc (Zn2+) for the purification of 6x histidine tagged proteins. This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalentzinc ion with a capacity of 20-40µmoles Zn2+/ml resin. The protein binding capacity is >20mg protein per ml resin. We have demonstrated binding of >40mg of a 50kDa 6XHis tagged proteins to a ml of resin. Immobilized Nickel, Cobalt andCopper Chelating Resins are also available. Cobalt has the highest selectivity followed by Zinc, Nickel then Copper, but has the lowest loading capacity. Copper has the highest loading capacity, followed by Nickel then Zinc. Specific binding/wash and elution buffers are available. Click here to request bulk or custom sizes.

Application:

Affinity purification ofzinc binding proteins Affinity purification of proteins with a 6x His tag.

Features:

For the purification of Zinc Binding proteins, including 6x His proteins High capacity: >20mg/ml Ligand density: 20-40µmoles Zn2+ /ml resin Bead Structure: 6% cross-linked agarose

Catalog#: 786-287
LID#: 4295P
Quantity :
Order by Phone or Fax: 1.800.316.3081
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Specifications

Warehouse Storage Temp4C
DescriptionImmobilized metal affinity chromatography (IMAC) resin utilizing zinc for the purification of 6x histidine tagged proteins and zinc binding proteins.
Unit Size10ml Resin
Safety StatementsFor Research Use Only
Sample AvailabilityYes
Synonymprotein purification, zinc resin, IMAC, immobilized metal affinity chromatography, zinc binding proteins, histidine, 6X His

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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