Yeast PE LB™ with Zymolyase®

Yeast PE LB™ with Zymolyase®

Product Information

Product Overview:

Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase ®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilizes a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase ® preparation is also provided. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Yeast PE LB™. Yeast PE LB™ has been tested on several widely used yeast strains. The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast PE LB™ eliminates the need for laborious glass bead lysis of yeast cells. This kit is suitable for processing 100 x 50µl yeast cell pellet preparations. Yeast PE LB™ buffer is also available separately for further downstream processing.

Application:

Lysis and extraction of proteins from yeast cells. Isolation of spheroplasts. For the isolation of biologically active proteins. Suitable for most applications including enzyme and protein purifications, electrophoresis, Western blotting and 2D-gel analysis.

Features:

Eliminates the need for glass bead lysis. Supplied as a kit, containing Zymolyase ® and reagents needed for protein extraction. Suitable for processing 100 x 50µl yeast cell pellet preparations.

Catalog#: 786-178
LID#: 4173P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionIsolate active proteins from yeast cells
Unit Size100 Preps
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymYeast PE LB, Yeast PELB, protein extraction, lysis buffer, yeast lysis buffer, spheroplast, 786-178, 786-179

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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