Product Overview:
The XIT™ Genomic DNA from Tissue kit is designed for the isolation of genomic DNA from fresh, frozen or methanol/ acetone fixed tissues. The kit uses three main steps (see figure) that are rapid & efficient cell lysis, enzymatic protein digestionand complete protein precipitation, followed by DNA precipitation. This yields high quality genomic DNA. The kit is supplied with three protocols for the isolation of: 1-10mg fresh or frozen tissue 50-100mg fresh or frozen tissue 5-10mg fixed tissue Three sizes of kit are available for processing a total of 0.25, 2.5 and 10g of tissue. The purified DNA has a A260/A280 ratio between 1.7 and 1.9 and is up to 200kb in size. The yield is approximately 0.5-10µg/mg tissue.
Features:
Isolate high quality genomic DNA Suitable for fresh, frozen and fixed tissue Yield 0.5-10µg/mg tissue Suitable for archive quality DNA.