Product Overview:
The XIT™ Genomic DNA fromGram Positive Bacteriakit is designed for the isolation of genomic DNA from overnight cultures. The kit uses three main steps (see figure) that are rapid & efficient enzymatic digestion of cell walls, spheroplast lysis and complete protein precipitation, followed by DNA precipitation. This yields high quality genomic DNA. Twosizes of kit are available for processing a total of 25 and 250ml of bacterial culture. The purified DNA has a A260/A280 ratio between 1.7 and 1.9 and is up to 200kb in size. The yield is approximately 15-25µg/ml culture.
Features:
Isolate high quality genomic DNA Suitable forGram positive bacteria Yield 15-25µg/ml bacterial culture Suitable for archive quality DNA.