Product Overview:
The XIT™ Genomic DNA fromGramNegative Bacteriakit is designed for the isolation of genomic DNA from overnight cultures. The kit uses three main steps (see figure) that are rapid & efficientbacteriallysis, complete protein precipitation, followed by DNA precipitation. This yields high quality genomic DNA. Twosizes of kit are available for processing a total of 25 and 250ml of bacterial culture. XIT™ Genomic DNA fromGram Negative BacteriaKit protocol is designed to use 1ml overnight culture, however the protocol can be easily adapted for larger tissue sample sizes. The purified DNA has a A260/A280 ratio between 1.7 and 1.9 and is up to 200kb in size. The yield is approximately 20-40µg/ml culture.
Features:
Isolate high quality genomic DNA Suitable for Gramnegative bacteria Yield 20-40µg/mg tissue Suitable for archive quality DNA.