Western ReProbe PLUS

Product Information

Product Overview:

Based on our popular Western ReProbe™, the modified formulation allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies. Each 100ml of Western ReProbe™ PLUS is sufficient for 25-30 standard size (7.5cm x 8.5cm) western blots. Western ReProbe™ PLUS is not recommended for stripping color producing Western blots that use substrates such as TMB, chloronapthol and DAB.

Application:

Screen same blot with different antibodies Reprobe for housekeeping proteins Compare phosphorylated & total protein on the same blot

Features:

Remove high affinity antibodies Simply incubate the blot in Western ReProbe™ PLUS at room temp & wash No boiling, denaturants or SDS required Repeated analysis of Western blots with multiple probes Re-analysis & correction of unsatisfactory Western blots Conservation of hard-to-obtain test samples & reagents Time saving in not rerunning gels & transfer processes

Catalog#: 786-307
LID#: 4316P
Quantity :
Order by Phone or Fax: 1.800.316.3081
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Specifications

Warehouse Storage Temp4C
DescriptionRemove/Strip High Affinity Antibodies from Western Blots without Heating or Denaturing
Unit Size500ml
Safety StatementsFor Research Use Only
Sample AvailabilityYes
Synonymwestern blot, western blotting, western blots, westernblot, immunoblot, western blot protocol, western blot detection, western protein, membrane, membrane protein, PVDF, nitrocellulose, antibody, stripping

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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