Western ReProbe™ [5X]

Western ReProbe™ [5X]

Product Information

Product Overview:

Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse western blots. Western ReProbe™ breaks the antigen-antibody binding affinity without using denaturants, SDS or boiling. The membrane-bound protein is retained on the membrane, and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane-bound protein is free to accept new proteins. Removal of antibodies also removes corresponding chemiluminescent or radio-isotopic signals from the blots. The stripped blots can then be probed with new probes (see figure 1). Western ReProbe™ is not recommended for stripping color-producing western blots that use substrates such as TMB, chloronapthol and DAB. Western ReProbe™ is supplied as a 5X concentrated solution. Each 100 milliliters is sufficient for 25 to 30 standard size (7.5cm x 8.5cm) western blots. For high affinity antibodies, we recommend our Western ReProbe™ PLUS.

Application:

Screen same blot with different antibodies Reprobe for housekeeping proteins Compare phosphorylated & total protein on the same blot

Features:

Simply incubate the blot in Western ReProbe™ at room temp & wash No boiling, denaturants or SDS required Repeated analysis of Western blots with multiple probes Re-analysis & correction of unsatisfactory Western blots Conservation of hard-to-obtain test samples & reagents Time saving in not rerunning gels & transfer processes

Catalog#: 786-306
LID#: 4315P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionRemove (Strip) and Re-probe Western Blots without Heating or Denaturing
Unit Size1L
Safety StatementsFor Research Use Only
Sample AvailabilityYes
Synonymwestern blot, western blotting, western blots, westernblot, immunoblot, western blot protocol, western blot detection, western protein, membrane, membrane protein, PVDF, nitrocellulose, antibody, stripping

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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