Toothpick™-PCR

Product Information

Product Overview:

Toothpick™-PCR is an extension of Toothpick™ and allows for the rapid release of plasmids from transformed bacteria for screening by polymerase chain reaction (PCR). There is no requirement for growing bacteria, performing "minipreps" or purifying the plasmid DNA. Add your colony directly to the supplied Toothpick™ solution, heat and then transfer 1µl directly to a PCR reaction to screen for your plasmid. Following PCR, add the supplied GLOW™ Loading Dye and run on an agarose gel. No need to stain with ethidium bromide, simply place gel on UV box and view. Glow Dyes™ have been specifically formulated for nucleic acid electrophoresis and contain an optimized concentration of buffer agents, tracking dyes and ethidium bromide. These loading dyes reduce exposure and many of the problems associated with ethidium bromide use and disposal. Figure: Screening of Plasmids with Toothpick™-PCR. Four transformed bacterial colonies were picked, resuspended in 15µl Toothpick™ solution and boiled for 5 minutes. 1µl was added to a 50µl standard PCR reaction and the resulting PCR products were resolved on an agarose gel. Lanes 1 and 2 show the correct plasmid.

Application:

For the rapid screening of colonies using PCR analysis.

Features:

Simple protocol, as easy as picking a colony. 5 minute protocol. No overnight cultures required. Enough reagent to screen 300 colonies by PCR. Supplied with GLOW™ Loading Dye to minimize ethidium bromide use.

Catalog#: 786-410
LID#: 4405D
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionRapid Colony Screening for Plasmid DNA with PCR
Unit Size300 Preps
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymToothPick-PCR, plasmid screening

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

Customer Reviews

Average Customer Rating
(0)
Review & Rating Distribution