Taq DNA polymerase

Taq DNA polymerase

Product Information

Product Overview:

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile, Thermus aquaticus. The molecular weight of the recombinant protein is 94kD.. The Taq polymerase is able to amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C. The error rate of this Taq polymerase is ~2.2x10-5 nucleotide-1 cycle-1. Taq DNA polymerase catalyzes the 5' ?3' synthesis of DNA. The enzyme has no detectable 3' ?5' proofreading exonuclease activity, and possesses low 5' ?3' exonuclease activity, which results in a 3'-dA overhang on the PCR product SOURCE A recombinant protein expressed in E. coli that carries the pol gene for Thermus aquaticus. STORAGEBUFFER 20mM Tris.HCl (pH8.0), 100mM KCl, 3mM MgCl2, 1mMDTT, 0.1% Nonidet ® P-40, 0.1% Tween ® 20, 0.2mg/ml BSA, 50% (v/v) glycerol. 10X PCR BUFFER 120mM Tris-HCl (pH8.8), 500mM potassium chloride, 1% Triton ® X-100, 100mM lysine and 25mM MgSO4 UNIT DEFINITION One unit (U) of Taq polymerase is defined as the amount of enzyme needed to catalyze the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70°C using herring sperm DNA as a substrate. Our Taq DNA Polymerase is offered in four convenient sizes and supplied with 10X PCR buffer (Mg2+ plus). Taq Polymerase 2X Mastermix is a ready-to-use mixture of our high quality Taq DNA polymerase, deoxynucleotides and reaction buffer at a 2X concentration.

Features:

5' ?3' polymerase activity No detectable 3' ?5' proofreading exonuclease activity Low 5' ?3' exonuclease activity Error rate ~2.2x10-5 nucleotide-1 cycle-1 Available as a 2X Mastermix

Catalog#: 786-447
LID#: 4427P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp-20C
DescriptionHighly stable DNA Polymerases
Unit Size1000U
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymTaq polymerase, PCR, polymerase chain reaction, amplification, Thermus aquaticus, PCR amplification, DNA labeling,DNA sequencing, PCR for cloning.

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: -20C

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