Superior™ Blocking Buffer in PBS (P...

Superior™ Blocking Buffer in PBS (Phosphate-buffered saline, pH 7.5)

Product Information

Product Overview:

Superior™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications. Superior™ Blocking Buffer is ideal for a high signal to background ratio in most system. Superior™ Blocking Buffer uses a non-serum protein and does not contain biotin or other animal source proteins to interfere with immuno-complexes. Superior™ Blocking Buffer is suitable for assays that use avidin/streptavidin systems. Superior™ Blocking Buffer for Precipitating Substrate is a modification of Superior™ Blocking Buffer. This blotting buffer has been optimized for used in blotting protocols that use precipitating substrates, such as TMB (3, 3', 5, 5'-Tetramethylbenzidine) and BCIP (5-Bromo-4-Chloro-3'-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrates. Superior™ Blocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining. Available in multiple formats using TBS, PBS, TBS with 0.05% Tween ®-20 or PBS with .05% Tween ®-20. Also supplied as a convenient dry form that is stable at room temperature. Each dry format pack is makes 200ml Superior™ Blocking Buffer.

Application:

For blocking Western blot membranes (PVDF and nitrocellulose) For blocking and storage of ELISA plates For blocking prior to immunohistochemistry staining Superior™ Blocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining.

Features:

Non serum protein blocking agent Rapid blocking times; ~2 minutes for ELISA Multiple formats; ready-to-use liquid (500ml) or dry buffer packs (for 5 x 200ml) Available with and without detergent (Tween ®-20)

Catalog#: 786-660
LID#: 4593I
Quantity :
Order by Phone or Fax: 1.800.316.3081
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Specifications

Warehouse Storage Temp4C
DescriptionFor high signal to background in Western blotting, ELISA and immunohistochemistry.
Unit Size500ml
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymBlocking buffer, blocking agent, non serum protein, ELISA, Western blotting, immunohistochemistry

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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