Superior™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications. Superior™ Blocking Buffer is ideal for a high signal to background ratio in most system. Superior™ Blocking Buffer uses a non-serum protein and does not contain biotin or other animal source proteins to interfere with immuno-complexes. Superior™ Blocking Buffer is suitable for assays that use avidin/streptavidin systems. Superior™ Blocking Buffer for Precipitating Substrate is a modification of Superior™ Blocking Buffer. This blotting buffer has been optimized for used in blotting protocols that use precipitating substrates, such as TMB (3, 3', 5, 5'-Tetramethylbenzidine) and BCIP (5-Bromo-4-Chloro-3'-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrates. Superior™ Blocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining. Available in multiple formats using TBS, PBS, TBS with 0.05% Tween ®-20 or PBS with .05% Tween ®-20. Also supplied as a convenient dry form that is stable at room temperature. Each dry format pack is makes 200ml Superior™ Blocking Buffer.

For blocking Western blot membranes (PVDF and nitrocellulose) For blocking and storage of ELISA plates For blocking prior to immunohistochemistry staining Superior™ Blocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining.