Product Overview:
The SpinOUT™ GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of protein and nucleic acid solutions ranging from 5µl to 4ml sample volumes. The SpinOUT™ columns are available in two MWCO sizes for >6,000 or >30,000 Dalton proteins and are suitable for samples containing as little as 20µg protein/ml. The SpinOUT™ columns are easy to use; simply apply the protein sample and centrifuge to recover proteins and nucleic acids with the column retaining more than 95% of the salts and small molecules (<1,000Da for GT-600 and <1,500Da for GT-1200). Spin-OUT™ GT-600 is for the purification of proteins >6kDa and nucleic acids larger than 10bp. Spin-OUT™ GT-1200 is for the purification of proteins >30kDa and removal of molecules >1,500Da. The columns are ideal for separating proteins from peptides. Five sizes are available for each MWCO and are highlighted below: Cat. # Description # Supplied Resin Bed Volume (ml) Sample Load Volume (ml) 786-703 SpinOUT™ GT-600, 0.1ml 25 0.1 0.005-0.02 786-170 SpinOUT™GT-600, 1ml 10 1 0.05-0.1 786-171 SpinOUT™GT-600, 3ml 10 3 0.1-0.5 786-704 SpinOUT™GT-600, 5ml 5 5 0.5-2 786-705 SpinOUT™GT-600, 10ml 5 10 0.5-4 786-706 SpinOUT™GT-1200, 0.1ml 25 0.1 0.005-0.02 786-172 SpinOUT™GT-1200, 1ml 10 1 0.05-0.1 786-173 SpinOUT™GT-1200, 3ml 10 3 0.1-0.5 786-707 SpinOUT™GT-1200, 5ml 5 5 0.5-2 786-708 SpinOUT™GT-1200, 10ml 5 10 0.5-4 For the desalting and clean up of PCR reactions, click here.
Application:
Protein purification and contamination removal from samples For the desalting and buffer exchange of protein and nucleic acid solutions Separate proteins from peptides
Features:
Offered in 2 MWCO sizes: >6kDa and >30kDa proteins Available in 5 sizes for sample volumes of 5µl to 4ml Easy-to-use, spin-format columns for rapid purification