SG-Chymotrypsin™

Product Information

Product Overview:

SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible. SG-Chymotrypsin™ is first treated with TLCK to inhibit trypsin that may be present and then subjected to an extensive purification process to remove contaminating protease and chymotryptic autolysis by-products. The highly purified enzyme is then chemically modified to increase its resistance to autolysis and stability. For protein digestion SG-Chymotrypsin™ is added to the protein at a ratio of 1:200 to 1:50, by weight, in a standard digestion buffer. Incubate at 25-30°C for 1 to 10 hours, but can be extended to 24 hours, due to the extended life of the SG-Chymotrypsin™. We recommend choosing a ratio of enzyme to protein that allows for the shortest incubation time possible. This will reduce or eliminate the catalyzed hydrolysis of peptide bonds with non-aromatic amino acid residues.

Application:

Digestion of proteins for sequence analysis. Suitable for sequencing applications.

Features:

Modified Chymotrypsin for sequence analysis. Resistant to autolysis and degradation. High Specificity, free from other known endopeptidases. Consistency of activity levels from lot to lot for reproducible digestions. Recommended SG-Chymotrypsin™ to protein ratio, 1:200 to 1:50.

Catalog#: 786-13
LID#: 4112E
Quantity :
Order by Phone or Fax: 1.800.316.3081
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Specifications

Warehouse Storage Temp4C
DescriptionHydrolysis of peptide bonds on the carboxy side of tyrosine, phenylalanzine and tryptophan
CAS#9004-07-3
Unit Size2 x 25µg
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymSG-Chymotrypsin, Chymotrypsin, sequencing grade, protease, protein sequencing

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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