SAM 265™: SAM Methyltransferase Ass...

SAM 265™: SAM Methyltransferase Assay

Product Information

Product Overview:

Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation (1). The S-adenosylmethionine (SAM) dependent methyltransferases use SAM, the second most commonly used enzymatic cofactor after ATP. SAM, also known as AdoMet, acts as a donor of a methyl group that is required for the modification of proteins and DNA. Aberrant levels of SAM have been linked to many abnormalities, including Alzheimer's, depression, Parkinson's, multiple sclerosis, liver failure and cancer (2). The SAM265:SAM Methyltransferase Assayis asensitive, UV-based, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases (3) without the use of radioactive labels or endpoint measurements. Figure 1 outlines the general scheme of the assay. Basically, the removal of the methyl group from SAM, by the SAM methyltransferase, generates S-adenosylhomocysteine, which is rapidly converted to S-ribosylhomocysteine and adenine by the included adenosylhomocysteine nucleosidase. This rapid conversion prevents the buildup of adenosylhomocysteine and its feedback inhibition on the methylation reaction. Finally, the adenine is converted to hypoxanthine by adenine deaminase, the second enzyme in the assay. The rate of production of hypoxanthine is measured by absorbance change. The assay can be adapted to be used with any SAM dependent methyltransferase or an enzyme reaction that produces 5-adenosylhomocysteine or 5'-methylthioadenosine, due to the specificity of adenosylhomocysteine nucleosidase. The kit is supplied with a positive control to determine the ideal assay conditions and with enough reagents for 100 x 115µlmicrowell assays. This kit is recommended for purified SAM-dependent methyltransferases.

Application:

For the kinetic analysis of protein SAM methyltransferase enzymes. Ideal for screening of methyltransferase inhibitors.

Features:

Detection of protein methylation or screening methylation inhibitors, using purified SAM-dependent methyltransferases Continuous enzyme coupled assay Supplied with all reagents, including positive control Adaptable for enzymes that generate S-adenosylhomocysteine or 5'-methylthioadenosine

Catalog#: 786-425
LID#: 4414A
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp-80C
DescriptionA sensitive, UV range, continuous enzyme coupled assay
Unit Size100 Assays
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymSAM Methyltransferase Assay, SAM265, adenosylmethionine, methylation, methylthioadenosine

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Dry Ice, (Handling Fee : $ 20.00 USD)

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: -80C

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