Product Overview:
Immobilized Protein A is for binding the constant domains of immunoglobulin (Ig) molecules (seeFigure 1). Protein A is coupled to agarose beads by reductive amination method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching (<5ng protein A/ml). Our immobilized protein A has a high binding capacity (>40mg/ml resin). The Immobilized Protein A 0.2ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples. Each 0.2ml column enables purification of 0.1-1mg IgG from 25-500µl of serum or other sample. The Immobilized Protein A 1ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples. Each 1ml column enables purification of 1-13mg IgG from 0.5-2ml of serum or other sample. Specific binding and elution buffers are also available. Click here to request bulk or custom sizes.
Application:
Suitable for immunoaffinity chromatography and immunoprecipitation
Features:
High binding capacity: >40mg human IgG/ml resin Ligand: Recombinant Staphylococcal Protein A lacking the albumin-binding domain produced in E. coli Bead size: 45-165µm Bead Structure: 4% highly cross-linked agarose