Product Overview:
The PhosphataseArrest™ phosphatase inhibitor cocktails are 100X concentrated, ready-to-use solutions that are simply added to your extraction buffers or samples. PhosphataseArrest™ is ideal for inhibition in tissue extractions and cell lysis experiments and is compatible with most common protein assays. In addition, PhosphataseArrest™ III is compatible with IEF/2D studies. PhosphataseArrest™ I A broad spectrum phosphatase inhibitor cocktail consisting of five phosphatase inhibitors that target all the phosphatase categories: serine/threonine (Ser/Thr) specific, tyrosine specific and dual specificity phosphatases A stabilized solution of sodium fluoride, sodium orthovanadate, sodium pyrophosphate, ß-glycerophosphate and sodium molybdate PhosphataseArrest™ II A phosphatase inhibitor cocktail consisting of five phosphatase inhibitors that target acid, alkaline and tyrosine phosphatases Contains optimized concentrations of sodium fluoride, sodium tartrate, sodium orthovanadate, imidazole and sodium molybdate PhosphataseArrest™ III A phosphatase inhibitor cocktail consisting of three phosphatase inhibitors that target both serine/threonine and alkaline phosphatases A stabilized solution of cantharidin, p-bromotetramisole oxalate and calyculin * If samples have high phosphatase activity then the PhosphataseArrest™ inhibitor cocktails can be used at two to three times concentrations to ensure complete inhibition.
Application:
Complete phosphatase inhibition in tissue extractions and cell lysis experiments Protection of protein phosphatase groups Compatible with most common protein assays Compatible with IEF/2D studies (PhosphataseArrest™ III)
Features:
A selection of 100X concentrated inhibitor cocktails Stabilized and ready-to-use No resuspension required