Product Overview:
The Nuclear & Cytoplasmic Extraction Kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies. This kit is based on organic buffers and contains a proprietary combination of various salts and agents. The kit is provided with reagents for solubilization of nuclear fraction. This kit is designed for >50 preps, depending on the tissue used, where one prep is 20x106 mammalian cells or 100mg mammalian tissue.
Application:
Extraction of cytosolic and nuclear protein fractions for transport studies, electrophoresis and other applications.
Features:
For generation of cytosolic and ultra pure nuclear fractions. Provided with nuclear protein solubilization buffers.