Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins. This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalent nickel ion with a capacity of 20-40µmoles Ni2+/ml resin. The protein binding capacity is >50mg protein per ml resin. We have demonstrated binding of >100mg of a 50kDa 6XHis tagged proteins to a ml of resin. The spin columns are supplied with a resin bed volume of 0.2, 1 and 3ml with total column volumes of 1, 8 and 22ml respectively. Columns can be used as a spin format of gravity flow columns. Immobilized Copper, Cobalt and Zinc Chelating Resins are also available. Cobalt has the highest selectivity followed by Zinc, Nickel then Copper, but has the lowest binding capacity. Copper has the highest binding capacity, followed by Nickel then Zinc. Specific binding/wash and elution buffers are available. Our new HOOK™ 6X His Protein Purification kits are now available and include everything required for purification of 6X His tagged proteins from yeast or bacteria, including lysis buffers, lytic enzymes, resin, columns and binding, wash and elution buffers. Each kit is available with nickel or cobalt chelating resins. Kits available are: HOOK™ 6X His Protein Purification (Bacteria): Isolation from bacteria; optimized to yield up to 10mg/250ml culture of soluble 6X His tagged protein. HOOK™ 6X His Protein Spin Purification (Bacteria): Isolation from bacteria;optimized to yield ~1mg/50ml culture of soluble 6X His tagged protein HOOK™ 6X His Protein Purification (Yeast): Isolation from yeast; optimized to yield up to 10mg of soluble 6X His tagged protein. HOOK™ 6X His Protein Spin Purification (Yeast): Isolation from yeast; optimized to yield up to 1mg of soluble 6X His tagged protein. Click here to request bulk or custom sizes.

Affinity purification of proteins with a 6x His tag.