Product Overview:
Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins. This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalent nickel ion with a capacity of 20-40?moles Ni2+/ml resin. Supplied as a kit that includes 12.5ml resin, 100ml binding/ wash buffer, 100ml elution buffer, 5 micro spin and 5 medi gravity flow columns.
Features:
For the purification of 6x His proteins
High capacity: >50mg/ml. Ligand density: 20-40µmoles Ni2+ /ml resin. Bead Structure: 6% cross-linked agarose.