Product Information

Product Overview:

A major drawback of current blocking solutions, such as BSA, casein and milk powder, is that they are derived from animal sources. The presence of animal proteins can often lead to high non-specific backgrounds as antigens and antibodies, generated in animals, interact with the "blocking " animal proteins. For improved assay sensitivity, minimal non-specific binding, and a high signal-to-background ratio, NAP-BLOCKER™, an animal-free protein blocking agent, is recommended. NAP-BLOCKER™ offers an alternative to milk-based blocking agents, minimizing the risk of non-specific binding of antibodies during the immuno-detection process and lowering the background. NAP-BLOCKER™ ensures no cross-reaction with your animal source antigens and antibodies, due to being 100% free of animal proteins. NAP-BLOCKER™ is easy to use and generates high publication quality blots (1-3). NAP-BLOCKER™ is free from biotin and other cross-reacting agents present in most animal source blocking agents. NAP-BLOCKER™ ensures uniform blocking without non-specific binding and shows improved results compared to milk powder preparations. NAP-BLOCKER™ is supplied as a pre-made, 2X concentrated solution; simply dilute with any buffer and block nitrocellulose or PVDF membranes. Alternatively, NAP-BLOCKER™ is supplied in PBS or TBS buffers.


Blocking agent for immunodetection assays Improved assay sensitivity & high publication quality blots Suitable for Western blot, dot blot & ELISA experiments


Animal-free, 2X concentrated solution Uniform blocking without non-specific binding Reduced background staining No cross-reactions with animal source antigens & antibodies High signal-to-background ratio

Catalog#: 786-190P
LID#: 4192I
Quantity :
Order by Phone or Fax: 1.800.316.3081


Warehouse Storage Temp4C
DescriptionNon-Animal Protein Blocking Buffer for Immunoblots and ELISA
Unit Size2 x 500ml
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymELISA, immunoassay, blocking buffer, western blot, animal free, animal-free, western blotting, blocking solution, NAP-BLOCKER, NAPBLOCKER, non-animal

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No


Shelf Life: 12 MONTHS

Storage Temperature: 4C

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