MegaLong™

Product Information

Product Overview:

The majority of genomic DNA extraction methods involve numerous physical manipulations, including mixing, pipetting, shaking, binding to resin, elution, which results in sheared DNA that may not be suitable for further analysis. MegaLong™ isolates high molecular weight genomic DNA from a variety of samples, including animal tissues, cultured cells, whole blood, bacterial and yeast. MegaLong™ uses Genomic Tube-O-DIALYZER™, a unique, patented micro dialysis device with a 0.45µm membrane that minimizes sample manipulation, one of the main reasons for DNA breakage. Nuclei are isolated under mild extraction conditions and genomic DNA is released by digestion of nuclear proteins with a highly active LongLife™ Proteinase K. The digestion is performed in the Tube-O-DIALYZER™ and after digestion the Tube-O-DIALYZER™ is inverted to dialyze away digested protein and other impurities leaving behind highly pure and fully hydrated genomic DNA. The fragile, high molecular weight genomic DNA can be stored in the Tube-O-DIALYZER™ to further minimize mechanical manipulation of the DNA. The DNA is suitable for Southern blot analysis, recovery of Lambda shuttle vectors from transgenic animals, PCR, analysis by pulsed-field electrophoresis or any application where genomic DNA is required. The kit is supplied in two sizes for the purification of either 25 or 50 x 1-100mg samples.

Application:

For DNA libraries, PCR amplification, Southern blot analysis, pulse-field electrophoresis, and other applications where >100kb DNA is required

Features:

Isolation of high molecular weight genomic DNA. For animal tissues, cultured cells, whole blood, bacterial and yeast. Uses patented genomic Tube-O-DIALYZER™ to minimize sample manipulation.

Catalog#: 786-146
LID#: 4143D
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionExtract high molecular weight genomic DNA with minimal shearing
Unit Size25 Preps
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymMegaLong, genomic DNA isolation, Tube-O-Dilayzer, shearing

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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