InGel™ Blue

Product Information

Product Overview:

The InGel™ Blue kit provides a complete set of reagents for the in gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie or fluorescent stained protein spots/bands. The kit has all the reagents for the in-gel digestion of proteins by the supplied, chemically modified Mass Spectrometry Grade Trypsin. Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues. Typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. Trypsin naturally undergoes autolysis, producing trypsin fragments that interfere with sequence analysis. Our Mass Spectrometry Grade Trypsin is a chemically modified trypsin that is enzymatically active and yet resistant to autolysis. It is methylated, TPCK treated and quality tested for mass spectrometry. Following trypsin digestion the kit allows for the extraction of the digested proteins from the gel pieces for analysis by mass spectrometry (MALDI and LC MS/MS). The InGel™ Blue kit is suitable for ~100 protein spots or bands. For silver stained proteins, we recommend InGel™ Silver

Application:

For MALDI Peptide Mass Mapping For LC MS/MS

Features:

For the in-gel tryptic digestion of proteins Compatible with Coomassie and fluorescent stained proteins Suitable for MALDI peptide mapping and LC MS/MS Supplied with Mass spectrometry grade trypsin Supplied with destining reagents and peptide extraction reagents

Catalog#: 786-681
LID#: 4612P
Quantity :
Order by Phone or Fax: 1.800.316.3081
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Specifications

Warehouse Storage Temp4C
DescriptionFor the in-gel tryptic digestion of Coomassie and fluorescent stained proteins
Unit SizeFor 100 Spots
Safety StatementsFor Research Use Only
Sample AvailabilityYes
Synonymtrypsin digestion, ingel, in-gel, ingel blue, peptide extraction, MALDI, mass spectrmetry, mass spec

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Blue Ice, (Handling Fee : $ 10.00 USD)

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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