Immobilized Protein G Resin

Immobilized Protein G Resin

Product Information

Product Overview:

Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of Streptococcal group G so that it does not bind to albumin. Protein G is coupled to 4% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for Ig and minimal protein G leaching. Our immobilized protein G has a high binding capacity (>20mg sheep IgG/ml resin). Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA. The Immobilized Protein G 0.2ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples. Each 0.2ml column enables purification of 0.1-1mg IgG from 25-500µl of serum or other sample. The Immobilized Protein G 1ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples. Each 1ml column enables purification of 1-13mg IgG from 0.5-2ml of serum or other sample. Specificbinding and elution buffersare also available. Click here to request bulk or custom sizes.

Application:

Suitable for immunoaffinity chromatography and immunoprecipitation.

Features:

High binding capacity: 38mg human IgG/ml resin; >20mg sheep IgG/ml resin Ligand: Recombinant Streptococcal Protein G lacking the albumin-binding domain produced in E. coli Bead size: 50-165µm Bead Structure: 4% highly cross-linked agarose

Catalog#: 786-284
LID#: 4292P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionFor the purification of antibodies through the constant IG domains.
Unit Size5ml Resin
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymImmobilized Protein G, IgG, immunoglobulin, antibody purification, immunoprecipitation, immunoaffinity

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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