HOOK™ HRP SULFO Labeling

HOOK™ HRP SULFO Labeling

Product Information

Product Overview:

G-Biosciences' HOOK™ Enzyme Labeling Kits are designed for the high efficiency coupling of horseradish peroxidase (HRP) to proteins, particularly antibodies. Our HOOK™ HRP SULFO Labeling Kit is an efficient enzyme labeling kit for tagging proteins with horseradish peroxidase (HRP) enzyme. The pre-activated HRP couples to peptides, proteins and ligands that have free sulfhydryl groups. The maleimide-activated HRP saves hours of valuable research time, as the first step of the normal two-step maleimide activation procedure is already complete. To aid in the preparation of HRP conjugates using free sulfhydryls, the kit is supplied with SATA (N-Succinimidyl S-acetylthioacetate), to add free sulfhydryls to existing amine groups, and 2-mercaptoethylamine·HCl (a mild reducing agent) for conjugating HRP to immunoglobulin G (IgG) and its fragments. The HOOK™ HRP SULFO Labeling Kit is supplied with G-Biosciences' Tube-O-Reactor™, a system that allows all the key steps of enzyme labeling of proteins to be performed in a single tube. This minimizes the risk of sample loss, experimental time and hands-on phases. Enzyme labeling involves three main steps that can all be performed in the supplied Tube-O-Reactor™: 1. Equilibration of reaction conditions for optimized reactions 2. Subsequent reaction of maliemide activated enzymes with target proteins 3. Removal of unreacted agents and by-products

Application:

Couple HRP to antibodies & other proteins for Western Blotting & ELISA

Features:

Reacts with free sulfhydryls to form covalent bonds Supplied with reagents to generate sulfhydryl groups

Catalog#: 786-314
LID#: 4321P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionAn efficient enzyme labeling kit for tagging proteins with horseradish peroxidase (HRP) enzyme
Unit Size5 Reactions
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymHOOK HRP Sulfo LABELING KIT, maleimide, horseradish peroxidase, enzyme labeling, antibody labeling

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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