G-Biosciences' HOOK™ Enzyme Labeling Kits are designed for the high efficiency coupling of horseradish peroxidase (HRP) to proteins, particularly antibodies. Our HOOK™ HRP SULFO Labeling Kit is an efficient enzyme labeling kit for tagging proteins with horseradish peroxidase (HRP) enzyme. The pre-activated HRP couples to peptides, proteins and ligands that have free sulfhydryl groups. The maleimide-activated HRP saves hours of valuable research time, as the first step of the normal two-step maleimide activation procedure is already complete. To aid in the preparation of HRP conjugates using free sulfhydryls, the kit is supplied with SATA (N-Succinimidyl S-acetylthioacetate), to add free sulfhydryls to existing amine groups, and 2-mercaptoethylamine·HCl (a mild reducing agent) for conjugating HRP to immunoglobulin G (IgG) and its fragments. The HOOK™ HRP SULFO Labeling Kit is supplied with G-Biosciences' Tube-O-Reactor™, a system that allows all the key steps of enzyme labeling of proteins to be performed in a single tube. This minimizes the risk of sample loss, experimental time and hands-on phases. Enzyme labeling involves three main steps that can all be performed in the supplied Tube-O-Reactor™: 1. Equilibration of reaction conditions for optimized reactions 2. Subsequent reaction of maliemide activated enzymes with target proteins 3. Removal of unreacted agents and by-products

Couple HRP to antibodies & other proteins for Western Blotting & ELISA