Product Information

Product Overview:

G-Biosciences' HOOK™ Enzyme Labeling Kits are designed for the high efficiency coupling of horseradish peroxidase (HRP) to proteins, particularly antibodies. Our HOOK™ HRP PLUS is designed for tagging proteins with the horseradish peroxidase enzyme. The pre-activated HRP couples with high efficiency (>90%) to the numerous amine groups of proteins, a superior method to the commonly used glutaraldehyde-coupling chemistry. The HRP has been activated by the addition of reactive aldehydes. The aldehyde groups react spontaneously and at high efficiency with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base complexes. These, in turn, are selectively reduced by the supplied reduction agent. Following quenching of the reaction, the protein is linked to the horseradish peroxidase enzyme by stable amine linkage. The labeled protein or antibody can now be used for immunoblotting, ELISA and histochemical techniques. The HOOK™ HRP PLUS Labeling Kit is supplied with our ready-to-use JAW™ Dry Buffer Packs for convenient PBS and Carbonate/Bicarbonate buffer preparation. The activated HRP PLUS is also available separately (reagent only).


Couple HRP to antibodies &other proteins for Western Blotting & ELISA


Activity is 120-200 units/mg Reacts with primary amines to form covalent amine bonds Stable for >12 months at -20°C High consistency

Catalog#: 786-313
LID#: 4320P
Quantity :
Order by Phone or Fax: 1.800.316.3081


Warehouse Storage Temp4C
DescriptionA high efficiency enzyme labeling kit for tagging proteins with horseradish peroxidase enzyme
Unit Size5 Reactions
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymHOOK HRP PLUS LABELING KIT, horseradish peroxidase, periodate, schiff base, antibody labeling, enzyme labeling, HOOK Enzyme Labeling

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No


Shelf Life: 12 MONTHS

Storage Temperature: 4C

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