HOOK™ GST Protein Spin Purification...

HOOK™ GST Protein Spin Purification Kit (Yeast)

Product Information

Product Overview:

HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase ® to release total soluble protein, whilst maintaining the structure and activity of the protein. The GST tagged protein is purified by affinity chromatography by adding 0.5ml Glutathione resin to the clarified lysate. The resin is transferred to a convenient spin column, where it is rapidly washed and the GST protein is eluted. Yeast-PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase ® based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase ® preparation is also provided. Depending on application, additional agents such as protease inhibitors may be added into Yeast PE LB™. The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast PE LB™ eliminates the need for laborious glass bead lysis of yeast cells. HOOK™ GST Protein Spin Purification kit is optimized to yield ~1mg of soluble GST tagged protein, with a purity of 80-90%, dependent on expression levels, resin type, conformation and solubility characteristics of the protein. For larger scale GST protein isolation from yeast we recommend our gravity flow kits for >10mg protein from yeast.

Application:

Spin column format for the isolation of GST-tagged recombinant proteins from yeast

Features:

Isolate >1mg GST tagged proteins from ~50ml yeast culture No sonication required, supplied with lysis buffer and enzyme Spin column format

Catalog#: 786-642
LID#: 4579P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionFor the purification of GST-tagged proteins from yeast.
Unit Size25
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymGST tag, recombinant protein, glutathione resin, yeast, spin format

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

Customer Reviews

Average Customer Rating
(0)
Review & Rating Distribution