HOOK™ GST Protein Spin Purification...

HOOK™ GST Protein Spin Purification Kit (Bacteria)

Product Information

Product Overview:

HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from bacterial cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity of the protein. The GST tagged protein is purified by affinity chromatography by adding 0.5ml glutathione resin to the clarified lysate. The resin is transferred to a convenient spin column, where it is rapidly washed and the GST protein is eluted. Bacterial- PELB™ kit has been developed for the extraction of soluble proteins from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The Bacterial-PE LB™ lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal force. HOOK™ GST Protein Spin Purification kit is optimized to yield ~1mg/50ml culture of soluble GST tagged protein, with a purity of 80-90%, dependent on expression levels, resin type, conformation and solubility characteristics of the protein. For larger scale GST protein isolation from bacteria we recommend our gravity flow kits for >10mg protein from ~250ml bacteria.

Application:

Spin column format for the isolation of GST-tagged recombinant proteins from bacteria

Features:

Isolate >1mg GST tagged proteins from ~50ml bacterial culture No sonication required, supplied with lysis buffer and enzyme Spin column format

Catalog#: 786-640
LID#: 4577P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionFor the purification of GST-tagged proteins from bacteria.
Unit Size25
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymGST tag, recombinant protein, glutathione resin, bacteria, spin format

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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