HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase ® to release total soluble protein, whilst maintaining the structure and activity of the protein. The GST tagged protein is purified by passing the clarified lysate through prepacked columns. Yeast-PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase ® based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase ® preparation is also provided. Depending on application, additional agents such as protease inhibitors may be added into Yeast PE LB™. The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast PE LB™ eliminates the need for laborious glass bead lysis of yeast cells. HOOK™ GST Protein Spin Purification kit is optimized to yield up to 10mg of soluble GST tagged protein, with a purity of 80-90%, dependent on expression levels, resin type, conformation and solubility characteristics of the protein. For small scale, rapid GST protein isolation from yeastwe recommend our spin format kits.

For the isolation of GST tagged recombinant proteins from yeast