HOOK™ GST Protein Purification Kit ...

HOOK™ GST Protein Purification Kit (Yeast)

Product Information

Product Overview:

HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase ® to release total soluble protein, whilst maintaining the structure and activity of the protein. The GST tagged protein is purified by passing the clarified lysate through prepacked columns. Yeast-PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase ® based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase ® preparation is also provided. Depending on application, additional agents such as protease inhibitors may be added into Yeast PE LB™. The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast PE LB™ eliminates the need for laborious glass bead lysis of yeast cells. HOOK™ GST Protein Spin Purification kit is optimized to yield up to 10mg of soluble GST tagged protein, with a purity of 80-90%, dependent on expression levels, resin type, conformation and solubility characteristics of the protein. For small scale, rapid GST protein isolation from yeastwe recommend our spin format kits.

Application:

For the isolation of GST tagged recombinant proteins from yeast

Features:

Isolate >10mg GST tagged proteins from yeast No sonication required, supplied with lysis buffer and enzyme

Catalog#: 786-643
LID#: 4580P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionFor the purification of GST-tagged proteins from yeast using gravity flow
Unit Size5
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymGST tag, recombinant protein, glutathione resin, yeast

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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