HOOK™ GST Protein Purification Kit ...

HOOK™ GST Protein Purification Kit (Bacteria)

Product Information

Product Overview:

HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from bacterial cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity of the protein. The GST tagged protein is purified by passing clarified lysate through prepacked columns. Bacterial- PELB™ kit has been developed for the extraction of soluble proteins from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The Bacterial-PE LB™ lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal force. HOOK™ GST Protein Purification kit is optimized to yield up to 10mg/250ml culture of soluble GST tagged protein, with a purity of 80-90%, dependent on expression levels, resin type, conformation and solubility characteristics of the protein. For small scale, rapid GST protein isolation from bacteria we recommend our spin format kits.

Application:

For the isolation of GST tagged recombinant proteins from bacteria

Features:

Isolate >10mg GST tagged proteins from ~250ml bacteria No sonication required, supplied with lysis buffer and enzyme

Catalog#: 786-641
LID#: 4578P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionFor the purification of GST-tagged proteins from bacteria using gravity flow
Unit Size5
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymGST tag, recombinant protein, glutathione resin

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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