Glycoprotein Staining

Product Information

Product Overview:

For the highly sensitive detection of glycoproteins following gel electrophoresis (Figure 1) or protein transfer to nitrocellulose membranes (Figure 2). The kit uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied oxidizing agent first oxidizes the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive Glyco-Stain Solution forming Schiff bonds and producing strong magenta color bands. In addition to glycoprotein staining, the kit is supplied with RAPIDstain™, an enhanced Coomassie stain. RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining. The GlycoproteinStaining kit is highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included. In addition, the kit allows for the detection of glycosylated and non-glycosylated proteins on a single gel or membrane. The Glycoprotein Staining kit is supplied with an enhance Coomassie stain, RAPIDstain™, that enhances the glycoprotein staining as well as staining non-glycosylated proteins (Figure 1B). The kit is sufficient for 10 mini gels (8 x 8cm) or 20 nitrocellulose membrane (8 x 8cm).

Application:

Improved detection of glycoproteins Stain polyacrylamide gels or nitrocellulose membranes

Features:

Specific glycosylated protein staining with optional non-glycosylated protein staining Two stains in one kit Nanogram level sensitivity Stable periodate/Schiff staining

Catalog#: 786-254
LID#: 4264P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionFor Staining Protein Gels for Glycoproteins
Unit Size10 Mini Gels
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymGlycoprotein Staining Kit, PAS, Periodic Acid-Schiff,

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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