Product Overview:
Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~8mg GST/ml resin. Supplied as slurry in 20% ethanol. The resin, columns and binding/wash and elution buffers are available separately. Our new HOOK™ GST Protein Purification kits are now available and include everything required for purification of GST tagged proteins from yeast or bacteria, including lysis buffers, lytic enzymes, resin, columns and binding, wash and elution buffers. Kits available are: HOOK™ GST Protein Purification (Bacteria): Isolation from bacteria; optimized to yield up to 10mg/250ml culture of soluble GST tagged protein. HOOK™ GST Protein Spin Purification (Bacteria): Isolation from bacteria;optimized to yield ~1mg/50ml culture of soluble GST tagged protein HOOK™ GST Protein Purification (Yeast): Isolation from yeast; optimized to yield up to 10mg of soluble GST tagged protein. HOOK™ GST Protein Spin Purification (Yeast): Isolation from yeast; optimized to yield up to 1mg of soluble GST tagged protein. Click here to request bulk or custom sizes.
Application:
Affinity purification of proteins with a GST tag.
Features:
For the purification of GST proteins High capacity (~8mg/ml) Ligand Density: 7-15µmoles glutathione/ml Bead size: 50-160µm Bead structure: 4% cross-linked agarose 10 carbon spacer arm.