Product Overview:
GET™ Plasmid DNA Maxiprep isolates super-coiled plasmid DNA from 100-500ml E. coli cultures. The extraction method is based on alkaline lysis of bacteria and uses our high affinity GET™ Plasmid Maxi Columns to bind and purify plasmid DNA. The extracted plasmid is ready for further DNA manipulation, such as restriction enzyme digestion, ligation, transformation, transfection and sequencing. The yield is on average 100-500µg/ prep depending on the volume of bacteria and plasmid copy number.
Application:
For plasmid isolation from large bacteria cultures
Features:
For 100-500ml bacterial cultures Isolate up to 0.5mg ready-to-use plasmid DNA No toxic chemicals, no phenol, no hazardous waste Available as 10 or 20 preps