Fluoro™ Protease Assay

Fluoro™ Protease Assay

Product Information

Product Overview:

The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485nm and emission at 530nm to determine protease activity. The kit detects nanogram level of proteases present in the sample. The kit is supplied with our chemically stabilized MSG-Trypsin™ for use as a general protease control; however, other specific protease standard controls can be used. MSG-Trypsin™ is an ultra-pure trypsin from porcine pancreas, modified by methylation followed by TPCK treatment and is extremely resistant to autolysis. The kit components are sufficient for 1,000 assays in a microtiter plate format.

Application:

Quantitative fluorescence protease assay

Catalog#: 786-320
LID#: 4325A
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionA Fluorometric, Quantitative Protease Assay
Unit Size1000 Assays
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymFluoro Protease Assay, fluorescent protease assay

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Blue Ice, (Handling Fee : $ 10.00 USD)

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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