Fluorescent ß-Galactosidase Assay (...

Fluorescent ß-Galactosidase Assay (MUG)

Product Information

Product Overview:

The Fluorescent ß-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the ß-galactosidase activity in the lysates of cells transfected with a ß-galactosidase expression construct The study of the lac operon has played an important role in understanding the control of gene expression in bacteria. In prokaryotes, gene expression is controlled primarily at the level of transcription. For eukaryotes, the promoter activity can be analyzed by using fusion genes containing the promoter of interest attached to the bacterial ß-galactosidase gene and be assayed by measuring ß-galactosidase activity. Because ß-galactosidase has a high turnover rate and is absent in mammalian cells it serves as a very useful and sensitive reporting tool for gene expression. Esters of the fluorescent compound, 4-methylumbelliferone (4-MU), provide a sensitive, quantitative assay for ß-galactosidase. 4-methylumbelliferyl-ß-D-galactopyranoside (4-MUG) is a substrate of ß-galactosidase that does not fluoresce until cleaved by the enzyme to generate the fluorophore 4-methylumbelliferone. The assay can be used with extracts from different expression systems including mammalian, insect cells, yeast, and bacteria. The Fluorescent ß-Galactosidase Assay (MUG) provides a 96 well assay format for galactosidase activity that is suitable for high throughput applications. The production of the fluorphore is monitored at an emission/excitation wavelength of 365/460nm. This kit is designed for 500 micro-well assays.

Application:

For the detection of transfection levels in cells

Features:

Sensitve, fluorescent ß-galactosidase assay Uses 4-methylumbelliferyl-ß-D-galactopyranoside (4-MUG) fluorophore Supplied with 4-methylumbelliferone (4-MU) for calibration and standards 500 microassays

Catalog#: 786-654
LID#: 4587A
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionA highly senstive, fluorescent assay for the reproter gene ß galactosidase.
Unit Size500 Assays
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymMUG, beta-galactosidase, reporter, reporter gene assay,beta gal, b galactosidase

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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