FOCUS™ SubCell

Product Information

Product Overview:

FOCUS™ SubCell is designed for the total subcellular fractionation of cells and mammalian tissue into enriched fractions of nuclear, mitochondrial, cytosolic, and membrane proteins.The resulting mitochondrial fraction can be subsequently separated into heavy and light fractions by gradient centrifugation and the resulting mitochondria are >90% active and have intact inner and outer membranes.The kit includes reagents for optional steps that minimize contaminations of the nuclear fraction by cytoplasmic elements. FOCUS™ SubCell is suitable for cultured animal cells and can be adapted for animal tissues. The kit is designed for 50 preps, where one prep is equivalent to 2x107 mammalian cells or 100mg mammalian tissue.

Application:

Fractionation of cells and tissues into mitochondrial, nuclear, cytosolic and membrane proteins Suitable for proteomic and cell signalling studies

Features:

Adaptable for animal tissue Resulting fractions suitable for 2D electrophoresis and other applications Isolated mitochondria are ~90% active

Catalog#: 786-260
LID#: 4271P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionFor the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.
Unit Size50 Preps
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymFOCUS SubCell Kit, subcellular fractionation, mitochondria, nuclear, nuclei, membrane, fractions

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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