FOCUS™ Protein Reduction-Alkylation

FOCUS™ Protein Reduction-Alkylation

Product Information

Product Overview:

The FOCUS™ Reduction-Alkylation kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis. The disulfide bonds are reduced with a highly reactive and stable TCEP [Tris (2-carboxyethyl) phosphine] followed by blocking of the thiols by alkylation with iodoacetamide reagent preparation. The kit is supplied with proprietary buffers and reagents necessary for an efficient reduction and alkylation of the disulfide bridges while minimizing reoxidation of the thiols.

Application:

Preparation of protein samples for improved resolution on 2D gels. Reduction of disulfide bonds. Blocking of thiols by alkylation for preventing reoxidation of proteins.

Features:

2D electrophoresis compatible. Reduction and alkylation of protein samples in two simple steps. Supplied with a proprietary buffer to minimize reoxidation of the competing thiol pairs.

Catalog#: 786-231
LID#: 4241P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp4C
DescriptionImproves resolution and prevents streaking of protein spots on 2D gels.
Unit Size100 Preps
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymFOCUS Protein Reduction-Alkylation Kit, reduction, alkylation, TCEP, iodoacetamide, 2D electrophoresis

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: 4C

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