FOCUS™ Global Fractionation

FOCUS™ Global Fractionation

Product Information

Product Overview:

FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins. Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localized proteins have been shown to be involved in intracellular membrane trafficking and signaling. FOCUS™ Global Fractionation is designed for >50 preps, where one prep is: 100mg mammalian tissue 50µl wet animal cell pellet 50µl wet yeast pellet 50µl wet bacteria pellet 250mg plant tissue

Application:

Selective fractionation of membrane proteins from tissues, cells, plants, yeast, bacteria, insects, and other sources. Fractionation of membrane proteins from lipid rafts. Suitable for membrane protein preparation for 2D gel electrophoresis and other applications. Suitable for proteomics and cell signaling studies.

Features:

Fractionate integral, peripheral and lipid raft associated proteins. Fractionation of complex proteomes into multiple, specific fractions.

Catalog#: 786-018
LID#: 3970P
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage TempRT
DescriptionFractionation and enrichment of cytosolic and membrane signal, peripheral or integral proteins
Unit Size50 Preps
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymFOCUS Global Fractionation, cytosolic proteins, membrane proteins, signal proteins, peripheral proteins, integral proteins

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: RT

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