Destaining Solution for Reversible ...

Destaining Solution for Reversible Zinc Stain™[10X]

Product Information

Product Overview:

The Reversible Zinc Stain™ is a single step stain for the rapid detection of proteins resolved by PAGE (native gels or SDS denatured gels). No destaining is necessary. The stain is based on the interaction of Zinc ions with polyacrylamide and proteins. The stain works by depositing a zinc metal precipitate in the gel which turns the gel opaque white, while the SDS coating on the proteins prevents the stains from binding to the proteins. A negative image of the gel is produced; clear protein bands are detected against a semi-opaque white polyacrylamide background. Protein bands are visualized in as little as 10-15 minutes. The sensitivity of the Reversible Zinc Stain™ is 0.1-0.5ng BSA protein and does not interfere with electroelution of proteins or alter their biological properties. Gels stained with the Reversible Zinc Stain™ can be destained in 5 minutes before the transfer or electroelution of proteins. This stain works with native as well as SDS denatured gels and gels containing glycine, tricine and a variety of primary amine containing buffers. A larger size of the destain for the Reversible Zinc Stain™ is offered for your convenience. Reversible Zinc Stain™ has a unique property that in some situations makes it far superior than the highly sensitive silver stains. Silver staining, although very sensitive, is known not to detect certain types of proteins, including glycoproteins. Reversible Zinc Stain™, having comparable sensitivity, is able to stain glycoproteins (Figure 1 & 2)and phosphoproteins (Figure 2). Figure 2 shows the difference in glycoprotein staining between a silver stain and Reversible Zinc Stain™. Phosvitin, a phosphoglycoprotein, was resolved on two gels and stained either with a silver stain or Reversible Zinc Stain™. The phosvitin was only detected with the Reversible Zinc Stain™.

Application:

Suitable for staining of protein gels including native and SDS denatured gels and gels containing Glycine and Tricine Staining suitable for subsequent protein elution, blotting, sequencing and mass spectroscopy

Features:

Reversible, simple, fast, and sensitive High contrast protein bands Able to detect proteins unable to be detected with silver stains, i.e. glycoproteins Works with native and SDS denatured gels and gels containing Glycine and Tricine Excellent for subsequent protein elution, blotting, sequencing and mass spectroscopy

Catalog#: 786-32DSZN
LID#: 4336B
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage TempRT
DescriptionStains Hard to Stain Proteins, including Glycoproteins & Phosphoproteins
Unit Size500ml
Safety StatementsFor Research Use Only
Sample AvailabilityYes
SynonymReversible Zinc Stain, glycoprotein, phosphoprotein, protein stain

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Ambient

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: RT

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