CasPASE™ 13 assay with Ac-LEED-AFC ...

CasPASE™ 13 assay with Ac-LEED-AFC substrate

Product Information

Product Overview:

The CasPASE™ Apoptosis assays are designed to monitor apoptosis by measuring various caspase (protease) activities, a key early indicator of apoptosis in mammalian cells. The assay provides a simple and easy to follow method that can be monitored with a fluorescence reader. The assay is based on the detection of cleavage of a synthetic substrate, which has 7-amino-4-trifluromethyl coumarin (AFC) at the C-terminal. When liberated from the peptide, AFC produces an optical change that exhibits a fluorescence spectral shift between the substrate-conjugate and the free dye. The AFC substrate is fluorogenic (detected at 510 to 550 nm with a fluorometer). During the reaction, the substrate releases AFC free dye and undergoes a fluorescence shift. The reaction is selectively and irreversibly inhibited by the peptide Z-VAD-FMK (fluoromethyl ketone). Comparison of the fluorescence from an induced/apoptotic sample with an uninduced control allows one to determine the fold-increase in protease activity. The Caspase activity can also be quantitated by using a standard curve established with an appropriate free dye. When measuring fluorescence, the assay reaction is excited at 380 to 400nm and emission is read at 510 to 550nm. The assays can be conveniently adapted for high-throughput 96-well format. The assay system may be used with purified enzyme preparations, cell extracts or tissue lysates. CasPASE™ assays are available for caspase enzymes 1 through 10 and 13. Each CasPASE™ assay kit is supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC), and the potent caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards. Colorimetric Caspase Assays are also available.

Application:

Monitor apoptosis by measuring various caspase activities in cells and tissues Use with purified enzyme preparations, cell extracts, and tissue lysates Characterization of caspase enzymes

Features:

Assays for caspase enzymes 1 through 10 and 13 are available Exhibit fluorescence spectral shift Conveniently adaptable for high-throughput 96-well format All kits supplied with enzyme specific AFC-substrate, free dye (AFC) & inhibitor Z-VAD-FMK

Catalog#: 786-206A
LID#: 4215A
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Warehouse Storage Temp-20C
DescriptionSensitive, Fluorometric Apoptosis Assay All-in-One Monitor Apoptosis with an All-in-One Fluorometric & Colorimetric Apoptosis Assay
Unit Size50 Assays
Safety StatementsFor Research Use Only
Sample AvailabilityYes
Synonymapoptosis kits, apoptosis assay, apoptotic assay, caspase apoptosis assay, caspase activity assay, cell apoptosis assay, caspase, apoptosis, apoptotic, cell apoptosis, apoptosis detection, apoptosis assay methods, apoptosis assay protocol, cell death

Shipping & Handling

Estimated Ship Time: 2 Business Days

Shipped In: Blue Ice, (Handling Fee : $ 10.00 USD)

Hazardous Material: No

Storage

Shelf Life: 12 MONTHS

Storage Temperature: -20C

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