Product Overview:
HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced
by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the
reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle
construction. p24 is used as a marker antigen for observing the patient’s condition after treatment,
as it indicates the amount of virus in the blood.
The product is prepared by immunizing rabbit with recombinant p24 protein which was
over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus, subtype B, and was highly purified by several steps of chromatography.
Using this antiserum in Western blotting, the bands of 24 kD, 55 kD and 41 kD corresponding
respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS
virus infected cells.
This product is a biotinylated IgG ([biotin]/[IgG] = 8.0) produced from the IgG fraction of rabbit
anti-p24 serum.