Product Overview:
DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. As such, it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation involves the addition of a methyl group to DNA — for example, to the number 5 carbon of the cytosine pyrimidine ring — in this case with the specific effect of reducing gene expression. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.
Applications
1) Immunocytochemistry (Figure below and Ref.1 & 2) (~50-100 fold dilution)
2) Immuno-blotting detection of DNA with 5-methylocytosine on nitrocellulose (Ref. 3 & 4) (~1000 fold dilution).